RECOMBINANT PLASMID DNA pGD-14 CONTAINING HYBRID GENE, INCLUDING NUCLEOTIDE SEQUENCE OF DEXTRAN-BINDING DOMAIN OF BETACOCCI, JOINED WITH HUMAN INTERFERON-GAMMA GENE THROUGH ACID-LABILE SPACER, DETERMINING BIOSYNTHESIS OF CHIMERIC PROTEIN STRUCTURE Russian patent published in 2012 - IPC C12N15/00 C12N15/62 C12N15/23 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and can be used to obtain recombinant human interferon-gamma gene. Plasmid DNA pGD-14 is constructed, which determines expression in Escherichia coli bacteria cells of a genetically engineered structure consisting of an N-terminal sequence of a dextran-binding domain of betaccoci and the human interferon-gamma gene, which are joined in a single reading frame through an aspartate-proline acid-labile spacer.

EFFECT: invention provides stable expression of the dextran-binding domain of betacocci and human interferon-gamma gene at 20% of the total protein of the transformed Escherichia coli cell.

3 dwg, 3 ex