METHOD OF PURIFICATION OF HUMAN RECOMBINANT GROWTH FACTOR VIIa Russian patent published in 2011 - IPC A61K38/36 C07K1/22 

FIELD: medicine.

SUBSTANCE: method of purification of human factor VIIa includes creation of affine sorbent, which includes immobilisation of antibodies to factor VIIa on insoluble matrix, sorption of factor VIIa on affine sorbent, further evolution of factor VII a and control of correctedness of its post-translation modification and conformation. Obtaining affine sorbent is achieved by optimal combination of NHS-activated Sepharose 4FF as hard base and combination of monoclonal antibodies, produced by clones 4F4 and 6B8 of cultivated hybridioma, selected by the highest degree of output of monoclonal antibodies to epitopes of calcium-binding domain in composition of factor VIIa. Control of correctness of post-translation modification and conformation of factor VIIa is performed by immunoassay with application of boards covered with monoclonal antibodies, produced by clone 5D9. 95%-binding of affine sorbent with factor VII a is achieved after 5 minutes of contact with one cycle of interaction with sorbent.

EFFECT: reduction of factor VII purification time and expenditures on said process.

4 ex, 4 dwg, 7 tbl