METHOD FOR PRODUCING HIGH-AFFINITY POLYCLONAL ANTIBODIES Russian patent published in 2013 - IPC C07K16/00 C07K16/06 G01N33/53 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention discloses a method for producing high-affinity polyclonal antibodies. The method involves the stages as follows: a) application of an antibody solution on an affine sorbent with an immobilised antibody at an excess molar quantity of antigen antigen-specific antibodies; b) removal of proteins and low-affinity antibodies; c) removal of the bound medium-affinity antibodies by means of a moderately chaotropic agent, such as LiCl, MgCl₂, glycine, d) elution of the high-affinity antibodies by means of the chaotropic agent, e) removal of the latter and f) if needed, removal of antibody aggregates. The method provides producing the antibodies affinity and specificity of which substantially exceeds those of the polyclonal antibodies included in the state of the art.

EFFECT: use of the antibodies prepared by the method according to the invention enables prominently increasing sensitivity and specificity of immunoassay techniques.

13 cl, 5 dwg, 1 tbl, 4 ex