METHOD FOR ISOLATING AUTOANTIBODIES OF IMMUNOGLOBULIN G SUBCLASSES TO IMMUNOREGULATORY CYTOKINE TUMOR NECROSIS FACTOR Russian patent published in 2018 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: invention relates to medicine and concerns a method for isolating subclasses of immunoglobulin G (IgG) autoantibodies against immunoregulatory cytokine tumor necrosis factor (TNF), which consists in obtaining a total IgG fraction by affinity chromatography using the Protein G Sepharose sorbent, followed by neutralization, integration and dialysis of IgG, purification of the resulting fraction of IgG from the TNF impurity. In this case, isolation of IgG subclasses is made from immunoglobulin pharmaceutical preparation for intravenous administration, after obtaining the IgG fraction, it is purified from the TNF impurity by ultrafiltration using centrifugal filters, then, step of isolating IgG3 antibodies and obtaining a mixture of IgG1, IgG2, IgG4 by affinity chromatography is carried out; then IgG1 antibodies are isolated by repeated negative affinity chromatography, followed by pooling and dialysis, then IgG2 antibodies are isolated by multiple negative affinity chromatography followed by pooling and dialysis, then IgG1 and IgG2 antibodies are purified from the IgG4 impurity by negative affinity chromatography, followed by isolation of specific autoantibodies to the TNF subclasses IgG1, IgG2, IgG3 by affinity chromatography, followed by neutralization, dialysis.

EFFECT: invention provides obtaining pure fractions of autoantibodies to TNF subclasses IgG1, IgG2 and IgG3.

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