METHOD OF MANUFACTURING ASSOCIATED VACCINE AGAINST COLIBACTERIOSIS, STREPTOCOCCOSIS AND ENTEROCOCCAL INFECTION OF CALVES AND PIGLETS Russian patent published in 2011 - IPC A61K39/116 A61K39/09 A61K39/108 

FIELD: medicine.

SUBSTANCE: from affected organs of calves and piglets isolated are epizootic strains of Escherichia coli, producing thermally labial, thermally stable and Shiga-like toxins, as well as of hemolytic strains of Streptococcus bovis and Enterococcus faecalis. Cultures of Escherichia coli, Streptococcus bovis and Enterococcus faecalis are separately grown on nutrient broth at temperature 37°C. Cultures of Escherichia coli are grown for 7 days and cultures of Streptococcus bovis and Enterococcus faecalis for 24 hours until concentration of 5-6 billion microbial bodies in 1 ml is achieved. After that grown cultures are separately inactivated with formalin to concentration of 0.3-0.4% during 14 days. After that cultures are mixed in equal volume, packed and sealed.

EFFECT: obtained vaccine possesses high immunogenicity and specificity, is harmless.

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