FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and agriculture. Method includes preparation of a sterile liquid nutrient medium containing a source of carbon, nitrogen, potassium dihydrogen phosphate and magnesium sulphate, inoculation of pure culture of fungus and its cultivation. As a genus representatives oyster fungus. As a carbon source is represented fructose in an amount of 1–3 % of the weight of the medium. Culturing is carried out during 5–7 days at temperature 26–28 °C with stirring at a rate of 100–140 RPM. Then glycerine is added up to 24–26 % by volume.
EFFECT: method allows to accelerate the process of preparing inoculating mycelium, increase storage life without reducing its life, form mycelium without formation of dense structures and stabilize the state of aggregation.
1 cl, 2 tbl, 5 ex
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