FIELD: chemistry.
SUBSTANCE: experimental sample of peptides is analysed via electrospray ionisation Fourier transform mass spectrometry. Presence of isomerised aspartate is indicated by peaks in the mass spectra, which correspond to marker fragments z10-57, c6+57 and b6+H2O. Use of the method enables to correctly identify isomerised aspartate in position 7 of the fragment of 1-16 beta-amyloid as a result of simultaneous use of two independently fragmentation methods.
EFFECT: advantage of said method is the use of low nanomolar concentrations of the initial isoforms and wide dynamic range of the measured concentrations.
2 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PEPTIDE SEQUENCING BY MASS-SPECTROMETRY AND DEFINING OF THEIR AMINO ACID SEQUENCES | 2011 |
|
RU2498443C2 |
| METHOD OF MASS-SPECTROMETRIC SEQUENCING OF PEPTIDES WITH THE PREFERENTIAL B-ION FORMATION | 2017 |
|
RU2650639C2 |
| PROTEOTYPIC PEPTIDE Q9Y4W6-02 AND METHOD OF SPECTROMETRIC ANALYSIS OF THE CONTENT OF AFG3-LIKE HUMAN PROTEIN ON ITS BASIS | 2012 |
|
RU2673551C2 |
| METHOD FOR QUANTITATIVE MULTIPLEX ANALYSIS OF ALPHA-2-MACROGLOBULIN, FETUIN A AND SERUM AMYLOID A1 AS INFLAMMATORY FACTORS IN BLOOD SERUM USING MALDI-TOF MASS SPECTROMETRY | 2022 |
|
RU2789503C1 |
| EXOGENIC-INDUCED ANIMAL MODEL OF ALZHEIMER DISEASE | 2012 |
|
RU2532525C2 |
| METHOD FOR DEVELOPING ALZHEIMER DISEASE CELL MODELS | 2013 |
|
RU2544957C2 |
| METHOD OF ADJUSTING CHROMATOGRAMS OF PEPTIDE MIXTURES | 2010 |
|
RU2444731C1 |
| SYPHILIS DIAGNOSTIC TECHNIQUE WITH USING METHOD OF DIRECT PROTEOMIC BLOOD SERUM PROFILING | 2008 |
|
RU2381505C1 |
| METHOD OF PRODUCING ANALYTICAL TEST SYSTEM FOR MULTIPLEX IDENTIFICATION AND QUANTITATIVE MEASUREMENT OF CONTENT OF PROTEINS OF INTEREST IN BIOLOGICAL SAMPLE BY CONTENT OF CORRESPONDING PROTEOTIPC MARKER PEPTIDES | 2013 |
|
RU2595835C2 |
| METHOD FOR STRUCTURAL CHEMICAL ANALYSIS OF ORGANIC AND BIOORGANIC COMPOUNDS BASED ON MASS-SPECTROMETRIC AND KINETIC SEPARATION OF IONS OF SAID COMPOUNDS | 2009 |
|
RU2402099C1 |
