FIELD: molecular diagnostics.
SUBSTANCE: invention relates to the field of molecular diagnostics, namely to the development of a method for molecular diagnostics of systemic inflammatory response syndrome (SIRS), including sepsis, by quantitative determination of alpha-2-macroglobulin, fetuin A and serum amyloid A1 in serum human blood using MALDI-TOF mass spectrometry technology. According to the present invention, for alpha-2-macroglobulin, the peptide VGFYESDVMGR is used as an isotope-labeled standard, for fetuin A, HTFMGVVSLGSPSGEVSHPR, and for serum amyloid A1, FFGHGAEDSLADQAANEWGR, and the stable oxygen isotope 18O is used as an isotope label.
EFFECT: enabling effective quantitative multiplex analysis of alpha-2-macroglobulin, fetuin A and serum amyloid A1 as inflammatory factors in blood serum using MALDI-TOF mass spectrometry.
5 cl, 2 dwg, 1 tbl, 5 ex
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