FIELD: medicine.
SUBSTANCE: described is method of obtaining population of stem cells, which come from dental follicle of human, named FENC (coming from follicles stem cells of embryonic nerve crest), which include: a) collection of follicle sac in sterile conditions, splitting, growing and grafting of initial culture; b) optional amplification; c) sorting out on FAC - sorter by the following stem characteristics: SSEA-4, TRA 1-60, TRA 1-81, OCT-4+; d) analysis of RNA with respect to positivity by transcriptional factors Nanog and Rex-1.
EFFECT: invention makes it possible to obtain homogenous population of stem cells.
10 cl, 6 ex
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