FIELD: medicine.
SUBSTANCE: method includes cultivation of a cell mixture containing cardiomyocytes and non-cardiomyocytes produced of stem cells, with the exception of human embryo cells, in a culture medium. A cell mixture is cultivated in the following conditions: (i) low glucose feed, (ii) one or more conditions selected from a group consisting of: the low calcium content; the low nutrient content; lactic acid feed; asparaginic/glutamic acid feed and pyruvic acid feed.
EFFECT: invention provides high purity and yield of cardiomyocytes from the cell mixture containing cardiomyocytes and non-cardiomyocytes produced of stem cells, with the exception of human embryo cells.
11 cl, 23 dwg, 1 tbl, 15 ex
| Title |
Year |
Author |
Number |
| METHOD FOR INDUCING DEATH OF PLURIPOTENT STEM CELLS AND DIFFERENTIATED CELLS DIFFERENT FROM CARDIOMYOCYTES |
2010 |
- Khattori, Fumijuki
- Fukuda, Kejiti
|
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| METHOD FOR PRODUCING MESENCHYMAL STEM CELLS OF HUMAN PLURIPOTENT STEM CELLS, AND MESENCHYMAL STEM CELLS PRODUCED BY THIS METHOD |
2009 |
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- Kang Khiun Dzae
- Li Eun Dzu
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|
RU2528250C2 |
| METHOD OF CONSTRUCTING MASS OF MYOCARDIAL CELLS AND USE OF MASS OF MYOCARDIAL CELLS |
2008 |
- Khattori Fumijuki
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|
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| METHOD OF PRODUCING MESENCHYMAL STEM CELLS FROM HUMAN PLURIPOTENT STEM CELLS AND MESENCHYMAL STEM CELLS OBTAINED ACCORDING TO THIS METHOD |
2020 |
- Kim, Ki Nam
- Choi, Sung Hyun
- Oh, Boram
- Choi, Mi Kyung
- Cho, Jun Kwon
|
RU2823729C1 |
| METHOD OF OBTAINING CARDIOMYOCYTAL MATRIX |
2010 |
- Eremeev Artem Valer'Evich
- Svetlakov Anatolij Vasil'Evich
- Bol'Shakov Igor' Nikolaevich
- Sheina Julija Igorevna
- Polstjanoj Aleksej Mikhajlovich
|
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| METHOD OF CARDIOMYOCYTE SELECTION WITH INTRACELLULAR MITOCHONDRIA USED AS INDICATOR |
2005 |
- Khattori Fumijuki
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|
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| METHOD FOR PREPARING PLURIPOTENT CELLS |
2009 |
- Kiselev Sergej L'Vovich
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- Shutova Marija Vladimirovna
|
RU2399667C1 |
| METHOD FOR OBTAINING ENDOTHELIOCYTES FROM HUMAN EMBRYONIC STEM CELLS (VERSIONS) |
2008 |
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|
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| MEASURING METHOD OF FLUORESCENCE INTENSITY OF VOLTAGE-SENSITIVE FLUORESCENT DYE |
2010 |
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- Satokh Ju-Suke
|
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| METHOD FOR INDUCING STEM CELL DIFFERENTIATION INTO DOPAMINERGIC NERVE PROCESSOR CELLS |
2020 |
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|
RU2800840C1 |
