FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention refers to a method for preparing pancreatic hormone-producing cells (versions). The presented method involves culturing human induced pluripotent stem (iPS) cells in a medium containing (+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl)cyclohexane carboxamide dihydrochloride; further, culturing the prepared cells in a medium containing (i) 6-[[2-[[4-(2,4-dichlorophenyl)-5-(4-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]nicotinonitryl and/or (ii) (2'Z,3'E)-6-brominedirubin-3'-oxime; further, culturing the prepared cells in a medium containing (i) 6-[[2-[[4-(2,4-dichlorophenyl)-5-(4-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]nicotinonitryl and/or (ii) (2'Z,3'E)-6-brominedirubin-3'-oxime and activin; further, forming cell mass of the prepared cell and culturing the suspended cell mass in a medium; further, culturing the prepared cells in a medium containing retinoic acid, dorsomorphin, 4-[4-(1,3-benzodioxol-5-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl]-benzamide and basic fibroblast growth factor; further, culturing the prepared cells. Implementing the declared invention uses no feeder cell, whereas the medium is serum-free.
EFFECT: invention can be applicable in a cell-based therapy in treating diabetes mellitus.
3 cl, 8 dwg, 3 ex
