METHOD FOR THE CULTIVATION OF LEPTOTRICHIA, RESIDENT BACTERIA IN THE ORAL CAVITY Russian patent published in 2012 - IPC C12N1/20 C12R1/01 

FIELD: biotechnology.

SUBSTANCE: invention method shall be implemented by inoculation of clinical material from incubation to the surface of a plate-like chromogenic medium Uriselect which is a non-selective agar medium rich in peptone and tryptophane, produced by Bio-Rad (France) by sectorial inoculation method. The culture plate shall be incubated in anaerobic and microaerophilic (5%-10% CO₂) conditions, t_opt =+35+37ºC, for 48-72 hours. If required, repeated inoculation of the grown colonies of Leptotrichia isolates shall be performed, wherein the passage shall be performed by hatching and/or by a sterile cotton pad. The passages shall be incubated in the same conditions. The method for Leptotrichia cultivation using the Uriselect medium enhances the efficiency of Leptotrichia isolates from the clinical material and enables presumable identification of Leptotrichia by substrate color changes, cut strain loss in the event of repeated passages. The cultivation efficiency of the difficult-to-cultivate Leptotrichia - oral microbiocenosis residents in a human oral cavity is over 30%, the method being easy to implement and does not require costly equipment.

EFFECT: increased efficiency of Leptotrichia cultivation using the Uriselect medium, significant cost reduction.

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