FIELD: chemistry.
SUBSTANCE: invention relates to medical microbiology and a method of differentiating Francisella tularensis subsp. mediasiatica bacteria. The method involves testing the analysed culture using an indicator and a substrate in form of discs with nitrocefin. The discs are first washed with distilled water and then placed on the analysed strains. Further, incubation is carried out at 37°C for 5-10 minutes. Results are determined from the colour of the discs. Francisella tularensis strains of the Central Asian subspecies do not change the colour of the discs, while strains of other subspecies colour the discs red.
EFFECT: invention can be used for rapid analysis when differentiating Francisella tularensis subsp mediasiatica.
1 dwg, 2 tbl, 2 ex
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