METHOD OF IN VITRO DETERMINATION OF GLYCEMIC INDEX OF FOODSTUFF Russian patent published in 2012 - IPC G01N33/66 

FIELD: medicine.

SUBSTANCE: method involves: (1) protein and fat content test of the analysed foodstuff; (2) homogenisation and fine dispersion of the analysed foodstuff to produce a foodstuff sample applicable for analysis; (3) digestion of said homogenised and fine-disperse sample in an amount sufficient to provide a standard amount of available carbohydrates, with a mixture of digestive enzymes for a fixed period of time to produce a digested sample; (4) processing of the entire digested sample immediately after said fixed period of time to terminate enzymatic reactions; (5) measurement of glucose and at least two sugars or sugar alcohols specified in a group consisting of fructose, galactose, lactose, sucrose and maltitol in the digested sample at the stage (4); and (6) calculation of a glycemic index of the analysed foodstuff on the basis of data prepared at the stages (1) and (5) with using an equation or a calculation method produced by the multiple analysis of the calibration data for sample calibration. Calibration data are referred to the same type and prepared by the same method as data for the analysed foodstuff prepared at the stages (1) and (5). The calibration samples have common in vivo values of the glycemic index. There are presented other versions of implementing the method.

EFFECT: higher determination accuracy and reliability.

20 cl, 4 ex, 36 tbl, 6 dwg