METHOD OF EVALUATING BACTERIAL LOAD OF URINE, PROSTATIC SECRETION, AND EJACULATE Russian patent published in 2012 - IPC C12Q1/04 

FIELD: medicine.

SUBSTANCE: bacterial load of substratum load is evaluated by sampling a material, taking its culture on selective nutrient mediums, recovering and identifying cultures. Urine, prostatic secretion, ejaculate cultures in sequential dilutions 10^-1 to 10^-10 are taken on Blaurock medium, Schadler broth, Schadler agar, blood agar (BA) on the basis of Muller-Hinton medium, bile-esculin agar for bacteroids - for cultivation of non-clostridial anaerobic bacteria. It is also combined with taking on Endo medium, chromogenic selective agar for enterococci, chromogenic selective agar for candida, chromogenic selective agar for Klebsiellas, chromogenic selective agar for staphylococci, BA on the basis of Muller-Hinton medium - for cultivation of optionally anaerobic bacteria. The optionally anaerobic and non-clostridial bacteria are detected with evaluating its concentration from minimal dilution level 10^-1.

EFFECT: method allows extending a spectrum of the recovered microbes in urine, prostatic secretion, ejaculate diagnosis and reducing time for bacteriologic examination.

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