METHOD FOR IDENTIFYING UROPATHOGENIC ENTEROCOCCUS FAECALIS IN CHILDREN Russian patent published in 2023 - IPC C12Q1/02 C12N1/20 C12R1/01 

FIELD: medical microbiology.

SUBSTANCE: invention relates to medical microbiology. A method for identifying uropathogenic Enterococcus faecalis isolated from urine in children with infections of the urinary system is proposed, including the selection of the test material, the isolation of pure cultures of microorganisms by inoculation on selective nutrient media and, when comparing the manifestations of biological properties, the determination of diagnostic biomarkers characteristic of etiologically significant or highly virulent strains of E. faecalis: for etiologically significant E. faecalis - fermentation of mannitol, lactose, its absence in relation to rhamnose, gelatin, the presence of hemolytic activity, sensitivity to second and third generation fluoroquinolones, detection of cylA and the absence of aac(6')-Ie-aph (2'')-Ia genes; for highly virulent strains - splitting of mannitol, lack of rhamnose fermentation and hemolytic activity, the presence of histodamaging substances, resistance to three classes of ABP (photoquinolones, aminoglycosides, sulfonamides), detection of aggA and absence of cylA genes.

EFFECT: method provides an assessment of the etiological significance of E. faecalis isolated from urine in children, and the adequacy of antibiotic therapy.

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