FIELD: chemistry.
SUBSTANCE: method of purifying apoliprotein A-1 involves mixing fractions of plasma IV, obtained via low temperature-ethanol fractionation with 1-8 M urea solution to form a prepared solution of fraction IV; feeding the prepared solution into a first column for anionic chromatography and subsequent elution with 1-8 M urea solution to obtain a solution of aroA-1 protein; and feeding the solution of aroA-1 protein into a second column for anionic chromatography and elution with 0-1 M urea solution to obtain pure aroA-1 protein.
EFFECT: higher purity.
26 cl, 7 dwg, 6 ex
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