METHOD FOR EXTENDED SCREENING OF PREDISPOSITION TO CARDIOVASCULAR DISEASES AND BIOCHIP FOR IMPLEMENTING SUCH METHOD Russian patent published in 2012 - IPC C12Q1/68 G01N33/48 

FIELD: medicine.

SUBSTANCE: method involves the stage of the recovery of genomic DNA from a clinical sample, the amplification of ACE, APOA4, APOA5, APOB, EDN1, MEF2A, FGA, ADRB1, TNBS4, APOE, FBN1, AGXT, MTHFR, CCR2, F5, F2, F7, ABCA1, ITGB3, AGTR2, B2R, DES, TLR4, NOS3, KDR, MMP9, THBS2, LPL, MPO gene sites containing sequences of polymorphic loci listed in SEQ ID 1-672 and the preparation of a one-chained fluorescent marked product by nick translation and restriction. A biochip applied for screening of predisposition to cardiovascular diseases and containing an immobilised oligonucleotide kit SEQ ID 1-672 is prepared. It is followed by hybridisation of the marked amplified product on said biochip. The results are recorded at wave length 635 nm and 532 nm for dyes Cy5 and Cy3 respectively. The hybridisation results are interpreted by comparing the intensity of fluorescent signals received after perfect and imperfect hybridisation.

EFFECT: invention allows producing an integrated assessment of patient's genetic predispositions to cardiovascular diseases and evaluating a risk of developing cardiovascular diseases.

6 cl, 29 tbl, 4 ex