METHOD FOR DIAGNOSING CONGENITAL CARDIAC ANOMALIES IN CHILDREN UNDER CONDITIONS OF EXCESSIVE NICKEL CONTAMINATION Russian patent published in 2025 - IPC G01N33/58 G01N33/52 G01N30/72 C12Q1/6806 C12Q1/6827 C12Q1/686 C12Q1/6876 C12Q1/6883 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to paediatrics, and can be used for diagnosing congenital cardiac anomalies in children under conditions of excessive nickel contamination. Child’s blood is examined for nickel, creatine phosphokinase (CPK) and Annexin V-FITC+7AAD-. MTHFR and IL-6 genes genotypes are analysed by polymerase chain reaction. When nickel content is 20% and more compared to the upper limit of the reference level equal to 7.5 mcg/dm³, at CPK level of 239 units/l and more, with content of Annexin V-FITC+7AAD- 11.8% and more, in the presence of the genotype CC of the MTHFR C677T (rs1801133) gene and genotype CC of the IL-6 G174C (rs1800795) gene, congenital heart anomalies are diagnosed in a child under conditions of excessive nickel contamination.

EFFECT: method provides accurate assessment of the effect of nickel on manifestation of congenital heart anomalies in children with excessive nickel contamination by determining the child’s blood nickel content, CPK level, Annexin V-FITC+7AAD- and genotypes MTHFR and IL-6.

2 cl, 2 tbl, 2 ex