METHOD FOR ASSESSING STATUS OF BLOOD VESSEL WALL Russian patent published in 2012 - IPC G01N33/49 

FIELD: medicine.

SUBSTANCE: patient's peripheral venous blood is sampled in the amount of 10 ml; patient's peripheral blood 5 ml is centrifuged at density gradient ρ=1.077, and another 5 ml of peripheral blood of the same patient are ultra-centrifuged. Mononuclear and endothelial cell fractions are placed in the related cultivation bottles. The cells adherent to the bottle surface are dissociated and counted up in a Goryaev chamber. The cell count is divided into a number of the counted up squares, multiplied by 1000 that corresponds to the content of the primary endothelial progenitor cells or the specialised endothelial progenitor cells at 5 ml of blood. If the primary endothelial progenitor cell count exceeds the specialised endothelial progenitor cell count in 20 times and more, the absence of endothelial dysfunction is stated - ED₀. If the primary endothelial progenitor cell count exceeds the specialised endothelial progenitor cell count in 5 to 20 times, high reparative ability of vascular endotheliocytes and preserving function of endotheliocytes, physiological endothelial dysfunction is stated, ED_f. If the primary endothelial progenitor cell count exceeds the specialised endothelial progenitor cell count in 2 to 5 times, a sufficient reparative ability of vascular endothelium and relative preserving function of endotheliocytes are stated, ED I. The primary endothelial progenitor cell count related to the specialised endothelial progenitor cell count as 1:1.0-1.4 or 1.5:1 shows moderate endothelial dysfunction and the preserving preparative ability of an endothelial layer - ED II. The primary endothelial progenitor cell count related to the specialised endothelial progenitor cell count as 1:≥1.5 enables to consider evident endothelial dysfunction with the severely impaired preparative ability of the endothelium - ED III.

EFFECT: use of the declared method allows both detecting the endothelial dysfunction, diagnosing a severity level, both assessing the repairative ability of the vascular wall that has a significant prognostic and therapeutic value.

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