FIELD: medicine; cardiology; neurology.
SUBSTANCE: blood sample is centrifuged at density gradient ficoll-verografin ρ=1.077 and the number of cast-off endotheliocytes per 100 lymphocytes is calculated in phase-contrast microscopy, with simultaneous identification of 100 lymphocytes by vesiculation of the external cytoplasmic membrane. The ratio of membrane-released microparticles is determined and the summing titer of antibodies to sPECAM-1 and phospholipids is calculated. The degree of endothelial dysfunction is diagnosed, depending on total titer of sPECAM-1 and antibodies to phospholipids.
EFFECT: enables determination of humoral markers by registration of cell markers, evaluation of degree of endothelial dysfunction, which provides early pathogenic therapy.
3 dwg, 8 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR EVALUATING LYMPHOCYTE EFFECT ON DEVELOPING ENDOTHELIAL DYSFUNCTION | 2011 |
|
RU2482487C2 |
| METHOD OF ENDOTHELIUM DYSFUNCTION DEGREE DIAGNOSTICS IN ESSENTIAL HYPERTENSION CASES, COMPLICATED BY ISCHEMIC STROKE | 2007 |
|
RU2331880C1 |
| METHOD FOR DETERMINATION OF DEGREE OF MANIFESTATION OF CARDIOVASCULAR PATHOLOGY BY CELL MARKERS | 2011 |
|
RU2461829C1 |
| METHOD OF PATIENTS CONDITION DIAGNOSTICS FOR HYPERTENSION CASES COMPLICATED BY ISCHEMIC STROKE | 2008 |
|
RU2349917C1 |
| METHOD FOR STIMULATING ENDOTHELIAL DYSFUNCTION IN VITRO | 2012 |
|
RU2490632C1 |
| DIAGNOSTIC TECHNIQUE FOR ENDOTHELIAL DYSFUNCTION IN PREGNANT WOMEN | 2020 |
|
RU2743851C1 |
| METHOD FOR ASSESSING STATUS OF BLOOD VESSEL WALL | 2011 |
|
RU2453845C1 |
| METHOD OF PREDICTION OF FROSTBITE DEGREE IN EARLY PERIODS | 2017 |
|
RU2646827C1 |
| METHOD FOR DETERMINING THE RISK OF DEVELOPMENT OF PURULENT-SEPTIC COMPLICATIONS AFTER RADICAL SURGICAL TREATMENT OF GASTRIC CANCER | 2022 |
|
RU2796243C1 |
| METHOD FOR PREDICTING CYTOPROTECTIVE EFFECT OF IMMUNOGLOBULINS FOR INTRAVENOUS ADMINISTRATION IN THERAPY OF FEMALES WITH RECURRENT PREGNANCY LOSS AND DIAGNOSED ANTI-PHOSPHOLIPID SYNDROME | 2014 |
|
RU2548754C1 |
