FIELD: medicine.
SUBSTANCE: there are offered a method and a kit for assessment of functional bypass activity of human complement component C9. The pharmaceutical preparation derinate is sorbed in microplate wells. Then the analysed sample containing the component C9 of unknown activity and porpoise complement is introduced in the analysed sample. It is followed with incubation, and after washing and drying of the dish, a conjugate of enzyme with component C9 antibodies and a substratum of this enzyme are added into the wells. The component C9 activity is evaluated by the amount of the prepared product of enzymatic reaction. The kit comprises the flat-bottomed microplate with sorbed derinate, conjugated enzyme and human complement component C9 antibodies, porpoise complement, substrate buffer and standard with known activity C9.
EFFECT: invention enables assessing the component C9 with using the preparation derinate as an classical activator of the complement.
2 cl, 1 dwg, 2 ex
