METHOD OF BIOTESTING ON SEED GERMINATION Russian patent published in 2013 - IPC G01N33/18 

FIELD: biotechnology.

SUBSTANCE: uniform stacking of seeds of round red radish with white tip is carried out on the filter paper in a Petri dish. 5 ml test sample of water is poured into the dish, and the liquid level in the dish must be lower than the surface of the seeds. At that the filter paper is taken of round shape with the diameter of the Petri dish with the mark on the edge, a pattern with holes for marking the places of planting seeds is placed on it. And one of the holes is oriented relative to the filter paper mark. Then the marked filter paper is placed in a Petri dish with the orientation of its mark in a northern direction on the compass, then the seeds are laid in the Petri dish on the marked places of planting on the filter paper. The water under study is poured, after germination of seeds prior to taking seedlings from the Petri dish for measuring the length of the root in each seedling, the angle of direction of its root is measured by compass as a factor of the azimuth of the root. In undergerminated seeds the azimuth of the root is taken as the absence of a quantitative value and a dash is put in the log of measurements. Subsequently, in all the seedlings in a Petri dish the length of the root is measured, and this length in the undergerminated seeds is taken as zero.

EFFECT: improvement of the method.

15 cl, 5 tbl, 1 ex