METHOD FOR VITRIFICATION OF BIOLOGICAL OBJECTS Russian patent published in 2012 - IPC C12N1/04 

FIELD: medicine.

SUBSTANCE: method involves vitrification of biological objects, preferentially gametes and embryos, in a flexible polycarbonate dropper for denudation and transfer of gametes and embryos and cumulus oocyte complexes of the diameter of 130-600 mcm and defrosting. A small amount of the vitrified solution free from biological objects is withdrawn in the dropper. Then the vitrified solution containing biological objects is withdrawn in the dropper, and the vitrified solution is added so than biological objects are at least at 10 mm from a distal end of the flexible polycarbonate dropper. The dropper is sealed from the distal end and checked for leaks by a Strippetor; a proximal end of the dropper is sealed. Then it is immersed in liquid nitrogen for min. 20 sec. and packed in a loaded outer casing culm of the min. volume of 0.25 ml for vertical orientation to be thereafter sealed. Defrosting is enabled by opening the outer casing culm, removing the sealed dropper with biological objects, placing the dropper in a waterbath at temperature 37 degrees Celsius. The dropper is opened from both sealed ends, and vitrified biological objects are tapped off into a defrosting solution with the use of a rubber bulb put on a wider portion of the dropper.

EFFECT: reduced price cost of the vitrification process and consumption of the frosting and defrosting solutions for biological objects.

6 dwg