FIELD: medicine.
SUBSTANCE: method involves blood sampling, erythrocyte separation and washing, incubation and centrifugation for preparing a supernatant. The supernatant is added with zinc chloride to the final concentration of 5-10 mM, mixed, and left for 60 minutes at room temperature. In 60 minutes, microvesicles are gravitated to the bottom of a test tube.
EFFECT: method provides simplifying the microvesicle process.
1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF ISOLATING ERYTHROCYTE MICROVESICLES | 2013 |
|
RU2558776C2 |
| METHOD OF MICROVESICULES INSULATION FROM BLOOD | 2017 |
|
RU2651521C1 |
| METHOD OF OBTAINING BLOOD EXOSOMES | 2016 |
|
RU2608509C1 |
| METHOD OF EXTRACTING FREE AND EXOSOMAL MICRO-RNA FROM SALIVA | 2018 |
|
RU2729423C2 |
| METHOD OF PRODUCING PURIFIED CULTURED BLOOD SERUM DEPLETED IN GROWTH FACTORS | 2023 |
|
RU2803918C1 |
| METHOD FOR ISOLATING EXOSOMES FROM CONDITIONED CELL CULTURE MEDIUM | 2020 |
|
RU2750928C1 |
| MEANS OF ERYTHROCYTE APOPTOSIS ASSESSMENT IN VITRO | 2015 |
|
RU2616498C2 |
| ANTHELMINTIC DRUG FROM THE GROUP OF BENZIMIDAZOLES | 2022 |
|
RU2798619C1 |
| METHOD FOR EVALUATING HEMOLYTIC ACTION OF POLYMERS, POLYMER MATERIALS AND ARTICLES MADE FROM THEM IN VITRO IN STATIC CONDITIONS | 2023 |
|
RU2818010C1 |
| METHOD OF EXTRACTION OF EXOSOMES FROM BLOOD | 2014 |
|
RU2556825C1 |
