FIELD: medicine.
SUBSTANCE: invention refers to microbiological industry and concerns the bacterium Escherichia coli that is a succinic acid producer, and a method for preparing succinic acid with the use of such bacteria. The described bacteria is modified by changing a nucleotide sequence of a promoter and a ribosome-binding site controlling expression of operon aceEF-lpdA genes in a bacterial chromosome so that expression of the aceE, aceF and lpdA genes in said bacteria is amplified. A method for preparing succinic acid consists in culture of such bacterium in a nutrient medium and recovery of succinic acid from a culture fluid.
EFFECT: invention provides producing a higher amount of succinic acid by microbiological procedure.
4 cl, 2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT BACTERIAL STRAIN Escherichia coli THAT IS SUCCINIC ACID PRODUCER (VERSIONS) AND METHOD FOR PREPARING SUCCINIC ACID WITH USING THIS STRAIN | 2012 |
|
RU2528056C2 |
| STRAIN OF BACTERIA ESCHERICHIA COLI-SUCCINIC ACID PRODUCER (VERSIONS) AND METHOD FOR PREPARING SUCCINIC ACID USING THIS STRAIN | 2015 |
|
RU2603004C1 |
| STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF FUMARIC ACID AND METHOD OF OBTAINING FUMARIC ACID USING THIS STRAIN | 2014 |
|
RU2573936C1 |
| METHOD OF OBTAINING L-AMINO ACIDS USING BACTERIA OF FAMILY ENTEROBACTERIACEAE | 2007 |
|
RU2364628C2 |
| MUTANT ACETOLACTASE SYNTHASE AND METHOD OF PRODUCING BRANCHED L-AMINO ACIDS | 2006 |
|
RU2355763C2 |
| METHOD OF PRODUCING L-threonine WITH USING BACTERIUM OF Escherichia TYPE | 2006 |
|
RU2351646C2 |
| METHOD OF PRODUCING L-AMINO ACIDS | 2007 |
|
RU2422530C2 |
| METHOD OF BUILDING (2S,3R,4S)-4-HYDROXY-L-ISOLEUCINE PRODUCING BACTERIA, (2S, 3R, 4S)-4-HYDROXY-L-ISOLEUCINE PRODUCING BACTERIA AND METHOD OF PRODUCING (2S, 3R, 4S)-HYDROXY-L-ISOLEUCINE OR SALT THEREOF | 2007 |
|
RU2395580C2 |
| MICROORGANISM AS PRODUCER OF L-AMINO ACID AND METHOD FOR PREPARING L-AMINO ACID (VARIANTS) | 2005 |
|
RU2316588C1 |
| MUTANT ADENYLATE CYCLASE, DNA CODING IT, BACTERIA OF ENTEROBACTERIACEAE FAMILY CONTAINING SAID DAN AND METHOD FOR PREPARING L-AMINO ACIDS | 2010 |
|
RU2471868C2 |
