STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF FUMARIC ACID AND METHOD OF OBTAINING FUMARIC ACID USING THIS STRAIN Russian patent published in 2016 - IPC C12N1/21 C12P7/46 C12N15/31 C12N15/67 

FIELD: biotechnologies.

SUBSTANCE: group of inventions relates to the strain of bacteria Escherichia coli FUM1.0 which is a producer of fumaric acid and to a method of obtaining fumaric acid using this strain. The strain is obtained by the inactivation of the genes ackA, pta, poxB, ldhA, adhE, pflB, frdA, frdB, sdhA, sdhB, aceA, aceB, and ptsG and amplification of the expression of the genes galP and glk in the strain Escherichia coli K-12 MG1655. The method of obtaining fumaric acid provides the cultivation of the named strain in suitable conditions with the subsequent release of fumaric acid from cultural liquid. The factor of conversion of glucose in fumaric acid at the cultivation of the offered strain amounts 1.31 mol/mol that is 65.5% of the theoretically possible maximum.

EFFECT: group of inventions provides the high yield of fumaric acid.

3 cl, 1 tbl, 2 ex