POLYEPITOPE PROTEIN, NUCLEOTIDE SEQUENCE CODING POLYEPITOPE PROTEIN, PLASMID WITH SEQUENCE CODING POLYEPITOPE PROTEIN, AND PREPARATION OF POLYEPITOPE PROTEIN FOR INDUCTION OF IMMUNE RESPONSE ON MURRAIN VIRUS Russian patent published in 2012 - IPC C07K14/09 C12N15/63 C07H21/00 A61K39/135 

FIELD: medicine.

SUBSTANCE: invention refers to a polyepitope vaccine protein applicable for immunisation against murrain virus, nucleic acid coding this protein, recombinant plasmid pA7248-AMV-H-PE for production of said protein in plants and to a vaccine preparation for prevention and protection against infection caused by murrain virus. The polyepitope vaccine protein has the amino acid sequence SEQ ID NO: 1 which contains the amino acid sequence of B-cell epitope of VP4 protein from 21-th to 40-th amino acid starting with N-terminal, the amino acid sequence of B-cell epitope of VP1 protein from 135-th to 160-th amino acid starting with N-terminal, the amino acid sequence of B-cell epitope of VP1 protein from 200-th to 213-th amino acid starting with N-terminal, the amino acid sequence of T-cell epitope of 2C protein from 68-th to 76-th amino acid starting with N-terminal, the amino acid sequence of T-cell epitope of 3D protein from 1-st to 115-th amino acid starting from N-terminal, and the amino acid sequence of T-cell epitope of 3D protein from 421-st to 460-th amino acid of murrain virus serotype O/ Taiwan/99. Nucleic acid coding such protein has the nucleotide sequence SEQ ID NO: 2. Recombinant plasmid pA7248-AMV-H-PE has a physical map presented in dwg. 5.

EFFECT: invention provides creating polyepitope vaccine protein which is applicable for immunisation against murrain virus.

5 cl, 7 dwg, 2 tbl, 6 ex