FIELD: chemistry.
SUBSTANCE: polyepitope proteins consisting of two or more protein fragments of the foot-and-mouth disease virus, particularly serotype O/Taiwan/99, connected by linker amino acid sequences, are constructed. The vaccine polyepitope protein can be characterised by general formula VP4(X1 -X2)-GRL-VP1(X3-X4)-GRL-VP1 (X5-X6)GRL-2C(X7-X8)-GRL-3D(X9-X10)-GRL-3D(X11-X12), where GRL is a glycine-rich linker, Xn-Xm are integers denoting the number of amino acid residues of the corresponding foot-and-mouth disease virus protein, VP4, VP1, 2C, 3D are names of foot-and-mouth disease proteins. The invention discloses a nucleotide sequence (NS) which codes peptides included in the polyepitope protein, a recombinant plasmid which facilitates synthesis of a hybrid polyepitope protein in procaryote (E.coli) and eucaryote (plant) cells, and a solvent composition for a foot-and-mouth disease vaccine based on the polyepitope protein.
EFFECT: polyepitope protein has high immunising power and can be considered a potential recombinant vaccine against foot-and-mouth disease.
15 cl, 7 dwg, 2 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| POLYEPITOPE PROTEIN, NUCLEOTIDE SEQUENCE CODING POLYEPITOPE PROTEIN, PLASMID WITH SEQUENCE CODING POLYEPITOPE PROTEIN, AND PREPARATION OF POLYEPITOPE PROTEIN FOR INDUCTION OF IMMUNE RESPONSE ON MURRAIN VIRUS | 2010 |
|
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| VACCINE COMPOSITION AGAINST TYPE A FOOT-AND-MOUTH DISEASE VIRUS | 2018 |
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|
SU1724691A1 |
| RECOMBINANT PLASMID DNA P9FMD, ENCODING HYBRIDOUS POLYPEPTIDE P199- ASP-PRO-CYS-CYS-VP1(200-213)-PRO-PRO-SER-PRO-VP1(131-152)-PRO-CYS-- GLY AND A STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF HYBRIDOUS POLYPEPTIDE P199-ASP-PRO-CYS-GLY-VP1(200-213)-PRO-PRO-SER -PRO-VP1(131-152)-PRO-CYS-GLY | 0 |
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| FMDV VACCINES BASED ON A RECOMBINANT ADENOVIRAL VECTOR AND USE THEREOF | 2017 |
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| O N 2620/ORENBURG/2021 STRAIN OF FOOT AND MOUTH DISEASE VIRUS APHTAE EPIZOOTICAE GENOTYPE O/ME-SA/IND-2001E FOR PRODUCTION OF BIOLOGICAL PRODUCTS FOR DIAGNOSIS AND SPECIFIC PREVENTION OF FOOT AND MOUTH DISEASE | 2023 |
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| O N 2241/Ethiopia/2011 STRAIN OF FMD VIRUS APHTAE EPIZOOTICAE GENOTYPE O/EA-3 FOR MANUFACTURE OF BIOPREPARATIONS FOR DIAGNOSIS AND SPECIFIC PREVENTION OF FMD | 2023 |
|
RU2809223C1 |
| STRUCTURE | 2010 |
|
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| APHTAE EPIZOOTICAE FOOT-AND-MOUTH DISEASE VIRUS STRAIN "O NO 2222/TAIWAN/1/2012" FOR PRODUCTION OF BIOPREPARATIONS FOR DIAGNOSIS AND SPECIFIC PREVENTION OF FOOT-AND-MOUTH DISEASE | 2023 |
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RU2817031C1 |
| O/KENYA/2017 STRAIN OF FMD VIRUS APHTAE EPIZOOTICAE GENOTYPE O/EA-2 FOR THE MANUFACTURE OF BIOLOGICAL PRODUCTS FOR THE DIAGNOSIS AND SPECIFIC PREVENTION OF FMD GENOTYPE O/EA-2 | 2022 |
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RU2793828C1 |
