METHODS OF DERIVING DIFFERENTIATED CELLS FROM STEM CELLS Russian patent published in 2016 - IPC C12N5/71 C12N5/797 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Disclosed method of producing definitive endoderm cells, which includes culturing stem cells derived from parthenogenetic cells in the presence of trichostatin A (TSA), in conditions where activin A is absent, where the TSA alters the epigenetic status of the cell; culturing the stem cells in the absence of TSA, thereby obtaining definitive endoderm cells, where the TSA is present in concentration of 100 nM-100 mcM and where culturing in the presence of TSA is carried out for 24-72 hours and culturing in the absence of TSA is carried out for 6-72 hours.

EFFECT: high efficiency of the method.

9 cl, 4 tbl, 3 dwg, 2 ex