PREPARING AND USING MONO- AND DI-PEGYLATED IL-10 Russian patent published in 2015 - IPC C07K14/54 A61K38/20 A61K47/48 A61P35/00 

Abstract RU 2549702 C2

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biotechnology, more specifically to a method for producing a mixture of mono- and di-pegylated IL-10, and can be used in medicine. The above method consists in carrying out a reaction of IL-10 protein in the concentration of 1 to 12 mg/ml with activated PEG-linker, wherein the relation of IL-10 and PEG-linker makes 1:1 to 1:7.7 in the presence of 25 to 35 mM of the reducing agent. The appropriate cases may require purifying the produced mixture of IL-10. The invention also refers to a pharmaceutical composition for treating a proliferative condition or disorder, containing a therapeutically effective amount of the mixture of mono- and di-pegylated IL-10 produced as described above, and a pharmaceutically acceptable carrier.

EFFECT: invention enables producing the mixture of mono- and di-pegylated IL-10 accompanied by no side products produced and keeping the protein dimer structure non-destructive.

16 cl, 2 dwg, 1 ex

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RU 2 549 702 C2

Authors

Blejzdell, Stiven, Dzh.

Katler, Kollett,M.

Paporello, Brittani, S.

Ambrozhelli, Aleksandr

Dates

2015-04-27Published

2009-12-15Filed