METHOD FOR FREEZE DRYING ERYTHROCYTE DIAGNOSTICUM Russian patent published in 2013 - IPC F26B5/06 

FIELD: chemistry.

SUBSTANCE: method for freeze drying an erythrocyte diagnosticum involves deep freezing the preparation, putting into a freeze drying chamber and further sublimation thereof in vacuum conditions with subsequent formation of a lyophilisate. At the preparatory step after deposition by centrifuging, separations of the supernatant fluid of erythrocytes are diluted in ratio of 1:9 by a drying medium consisting of a solution of 15 wt % rheopolyglucin and 7.5 wt % saccharose in distilled water, and shelf temperature and pressure in the sublimation chamber is not changed for the first 8 hours and maintained at -15°C and 30 Pa, respectively, and then for the next 14 hours, gradually varied at a rate of (2.6±0.4)°C/h and (2±0.1) Pa/h to 22°C and 3 Pa, respectively, and said parameters are maintained for at least 3 hours.

EFFECT: owing to the disclosed method of drying labile erythrocyte diagnosticums in maximally gentle conditions, activity thereof virtually does not change after prolonged storage and rehydration.

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