FIELD: biotechnologies.
SUBSTANCE: method includes the following stages: contact of a sample with a source of nutrition for cells, containing antioxidant, representing pyroracemic acid or its salt, and an inhibitor of cell proliferation, which is selected from ciprofloxacin and cefalexin; contact of the specified sample with fluorescent-marked oligonucleotide probes, capable of specific hybridisation at least with one section of ribosomal nucleic acids, which belong to microorganisms of Legionella pneumophila kind and type; and detection and quantitative determination of a fluorescent signal.
EFFECT: provided method and set make it possible to more accurately and reliably detect and calculate viable microorganisms of Legionella pneumophila type, having excluded natural fluorescence of microorganisms from calculation.
6 cl, 2 dwg, 6 tbl, 2 ex
| Title |
Year |
Author |
Number |
| METHOD OF DETECTING AND COUNTING VIABLE Legionella pneumophila MICROORGANISMS |
2009 |
- Fove Janik
- Djukre Adrian
- Dukan Sehm
|
RU2490327C2 |
| DETECTION AND CALCULATION OF MICROORGANISMS |
2011 |
- Fove, Jannik
- Djukre, Adrian
- Djukan, Sam
- Periam, Marina
|
RU2554470C2 |
| REVERSE TRANSCRIPTION METHOD COMBINED WITH MULTIPLEX PCR FOR VIDEO-SPECIFIC IDENTIFICATION OF CAUSATIVE AGENTS OF BACTERIAL AND VIRAL HUMAN PNEUMONIA WITH IMMOBILIZED PRIMERS AND A BIOLOGICAL MICROCHIP FOR ITS IMPLEMENTATION |
2021 |
- Lapa Sergej Anatolevich
- Miftakhov Rinat Azatovich
- Surzhikov Sergej Alekseevich
- Chudinov Aleksandr Vasilevich
|
RU2784653C1 |
| DIAGNOSING AND TREATING BREAST CANCER |
2011 |
|
RU2550925C2 |
| IMPROVED DETECTION OF SHORT HOMOPOLYMERIC REPEATS |
2016 |
|
RU2730622C2 |
| METHOD OF DETERMINING DNA FRAGMENTATION IN BACTERIA |
2007 |
- Gosal'Ves Berenger Khajme
- Fernendes Garsia Khose Luis
- Gojanes Vil'Jaehskusa Visente
- Bau Arevalo German
- Mur'El' Rios Lurdes
- Kartelle Gestal Monika
|
RU2420596C2 |
| METHOD OF DETECTION, IDENTIFICATION AND/OR QUANTITATIVE ESTIMATION OF MATERIAL, SET FOR ANALYSIS, OPTICAL WAVE GUIDE, BIOSENSOR |
1992 |
|
RU2116349C1 |
| IDENTIFICATION AND ANALYSIS OF MICROORGANISMS SAMPLES BY RAPID INCUBATION AND NUCLEIC ACIDS ENRICHMENT |
2020 |
|
RU2809853C2 |
| METHOD FOR SYNTHESISING A FLUORESCENTLY LABELLED DNA PROBE FOR A REACTION OF FLUORESCENT IN SITU HYBRIDISATION (FISH) |
2020 |
- Lagutinskaia Daria Vladimirovna
- Olkhovskii Igor Alekseevich
- Stoliar Marina Aleksandrovna
- Gorbenko Aleksei Sergeevich
|
RU2761308C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES FOR IDENTIFICATION AND SUBTYPING OF BACTERIAL DNA OF Pasteurella multocida OF SEROTYPES A, B, D, E, F BY PCR METHOD IN REAL TIME |
2014 |
- Shikov Andrej Nikolaevich
- Ternovoj Vladimir Aleksandrovich
- Nefedchenko Aleksej Vasil'Evich
- Glotova Tat'Jana Ivanovna
- Glotov Aleksandr Gavrilovich
- Sergeev Aleksandr Nikolaevich
- Agafonov Aleksandr Petrovich
|
RU2551958C1 |
