FIELD: biotechnologies.
SUBSTANCE: method includes depolymerisation of a high-molecular chitosan with hydrogen peroxide. The process of chitosan depolymerisation is carried out in a double-phase system. The solid phase is activated chitosan with Mav = 450-650 kDa and the average particle size of 0.05-0.20 mm. The liquid phase is a water solution of H2O2 with concentration of H2O2 in a reaction system equal to 1-7%. The reaction is carried out for 120-180 minutes at 70°C. Then phase separation of produced chitosan homologs is carried out by means of filtration via paper or textile surface of the produced reaction mixture. The produced filtrate contains water-soluble chitosan oligomers.
EFFECT: invention makes it possible to quantitatively control extent of conversion of an initial high-molecular chitosam into oligomer and low-molecular structures of its homologs.
1 tbl, 1 ex
Title | Year | Author | Number |
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PHARMACOLOGICAL AGENT POSSESSING ANTITUBERCULOSIS ACTIVITY | 2011 |
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RU2627870C1 |
METHOD FOR PREPARING CHITOSAN SOLUBLE FORMS | 2001 |
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RU2215749C2 |
METHOD OF PRODUCING LOW-MOLECULAR OLIGOMERIC CHITOSAN AND DERIVATIVES THEREOF | 2018 |
|
RU2703437C1 |
METHOD OF PRODUCING LOW-MOLECULAR CHITOSAN IN SALT-FREE MEDIUM VIA ENZYMATIC POLYMERISATION | 2009 |
|
RU2425844C2 |
ANTIPLATELET AGENT | 2017 |
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RU2647366C1 |
COMPOSITIONS OF PARTIALLY DEACETYLATED CHITIN DERIVATIVES | 2006 |
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RU2421467C2 |
Authors
Dates
2013-04-20—Published
2011-10-12—Filed