METHOD OF OBTAINING 2,4-DIHYDROXYBUTYRIC ACID Russian patent published in 2017 - IPC C12N9/02 C12N9/04 C12N9/12 C12P7/40 C12P7/42 C12N15/52 C12N15/63 

FIELD: chemistry.

SUBSTANCE: group of inventions refers to getting 2.4-dihydroxybutyric acid (2.4-DHB). A method of obtaining 2.4-DHB, which includes the first stage of the transformation of malate in 4-phosphomalate using an enzyme, capable to carry out such conversion, the second stage of the transformation of 4-phosphomalate in malate-4-half-aldehyde by using an enzyme capable to implement a similar metamorphosis, and third stage of transformation of malate-4-half-aldehyde in 2.4-DHB using an enzyme, capable to carry out such conversion. At the first stage, using an enzyme that has a sequence selected from the group consisting of SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 18, SEQ ID No. 20, SEQ ID No. 22, SEQ ID No. 24, SEQ ID No. 26, SEQ ID No. 39, SEQ ID No. 41, SEQ ID No. 43 and SEQ ID No. 45. The second stage uses an enzyme that has a sequence selected from the group consisting of SEQ ID No. 54, SEQ ID No. 56, SEQ ID No. 58, SEQ ID No. 60, SEQ ID No. 62, SEQ ID no. 64, SEQ ID No. 66 and SEQ ID No. 231. In the third stage, using an enzyme that has a sequence selected from the group consisting of SEQ ID No. 74, SEQ ID No. 76, SEQ ID No. 81, SEQ ID No. 225 and SEQ ID No. 227. Proposed options for the enzymes used in the method, nucleic acid variants which encode the relevant enzymes variants chimeric genes for transformation of the micro-organism-host and microorganism in the expression of the corresponding enzyme expression vector variants, variants of a micro-organism-host expressing the relevant enzyme.

EFFECT: improved method.

31 cl, 5 dwg, 16 tbl, 12 ex