FIELD: biochemistry.
SUBSTANCE: invention relates to the field of biochemistry. Disclosed is a method of extracting lipopolysaccharide Chlamydia trachomatis. Method involves extraction of lipopolysaccharide of bacterial cells with 90 % aqueous solution of phenol at 50 °C. Then supernatant is separated from undissolved components by centrifuging, diluted with 0.1 M tris-HCl buffer at a ratio of 1:9. Extraction of lipopolysaccharide Chlamydia trachomatis is carried out by affine chromatography on concanavalin-A-sepharose 4B. Extraction is followed by washing with 1 M NaCl solution, buffered with tris-HCl buffer, and elution with 0.4-0.45 M solution of N-acetyl-D-glucosamine in tris-HCl buffer with further dialysation and lyophilisation.
EFFECT: invention provides high degree of purity of extracted lipopolysaccharide.
1 cl, 4 dwg, 1 tbl
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