METHOD TO ASSESS BIOLOGICAL ACTIVITY OF LACTOBACILLI AND BIFIDUS BACTERIAL RELATIVE TO CHOLERAIC VIBRIOS IN VITRO Russian patent published in 2013 - IPC C12Q1/04 C12N1/20 

FIELD: biotechnologies.

SUBSTANCE: invention may be used to assess biological activity of lactobacilli and bifidus bacteria relative to Vibrio cholerae with the purpose to establish possibility to use probiotics for prevention and treatment of cholera. The method provides for testing of antagonistic and acid-forming activity of lactobacilli and bifidus bacteria strains. Antagonistic activity relative to choleraic vibrios is detected by the method of holes in dense nutrient media - CDM - agar and alkaline agar, and activity is accounted on the basis of quantitative data, namely: zone of growth inhibition (in mm) of a test strain V. cholerae from 6.0 to 15.0 - low antagonistic activity, from 15.1 to 29.0 - medium, ≥29.1 - high. Acid-forming activity of strains relative to choleraic vibrios expressed in Turner degrees (°T) is defined on the basis of quantitative data. So if acid formation ≤is 99.9 (°T), then activity of acid formation is assessed as low, if the values of this index are within (100-149.9) (°T) - as medium, and if the value is ≥150.0 (°T) - as high. At the same time the result of assessment of lactobacilli and bifidus bacteria biological activity relative to V. cholerae eltor, classica, O139, non O1/ non O139 is considered positive with 4 versions of combination of testing results according to the specified indices, namely: high antagonistic activity, high acid-forming activity, accordingly, high - medium, medium - high, medium - medium.

EFFECT: invention makes it possible to establish availability or absence of anti-cholera activity in strains.

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