FIELD: biotechnologies.
SUBSTANCE: method includes fixation, washing and dehydration, impregnation in camphene and drying at room temperature. Prior to fixation of the sample the microorganisms were cultivated on the surface of the sterilised cell of the cell package with perforations along the entire diameter of the cell submerged into the nutrient medium. The sample with microorganisms was fixed for 24 hours in the mixture of solutions of paraformaldehyde and glutaraldehyde on a phosphate buffer, pH 7.4, at the temperature of 4°C, and then washed in the phosphate buffer for 20 minutes and in distilled water for 6 minutes. Then the sample was dehydrated in stages in 70% ethyl alcohol for 15 minutes and for 10 minutes in 80%, 96% and 100% ethyl alcohol, impregnated in camphene at t=60°C for 20 minutes. Further samples are dried on air to full sublimation of camphene at room temperature.
EFFECT: prevention of deformation of cells and microrelief of cellular surface of bacteria, and spatial structure of microorganism microfilms and extracellular polymer matrix in process of drying.
3 dwg
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR CULTURING BACTERIAL BIOFILMS FOR OPTICAL MICROSCOPY | 2023 |
|
RU2824395C1 |
METHOD OF PREPARING BIOLOGICAL TISSUE SAMPLES FOR ANALYSIS IN SCANNING ELECTRON MICROSCOPE | 2008 |
|
RU2397472C1 |
METHOD FOR PREPARING LARGE SAMPLES OF REGENERATING BONE FOR SCANNING ELECTRON MICROSCOPY | 2020 |
|
RU2747640C1 |
METHOD FOR OBTAINING SAMPLES OF CHOLERA VIBRIO BIOFILMS FOR TRANSMISSION ELECTRON MICROSCOPY SURVEY | 2017 |
|
RU2662938C1 |
METHOD FOR PREPARING THE SURFACE OF BONE TISSUE SAMPLES TO STUDY THEIR MICROSTRUCTURE USING A SCANNING ELECTRON MICROSCOPE | 2017 |
|
RU2668879C1 |
METHOD OF PREPARING HUMAN BONE TISSUE SAMPLES FOR SCANNING BY ELECTRON MICROSCOPY | 2018 |
|
RU2688944C1 |
METHOD OF INTRAVITAL STUDY OF FUNCTIONING, REMODELING AND ENDOTHELISATION OF PROSTHESES FOR VASCULAR RECONSTRUCTION AFTER IMPLANTATION IN EXPERIMENT ON LARGE LABORATORY ANIMALS | 2023 |
|
RU2817271C1 |
METHOD TO DETERMINE FORMATION OF DEPOSITS IN AQUEOUS SYSTEM | 2006 |
|
RU2372400C2 |
ANTIBACTERIAL COATING ON ORTHOPAEDIC IMPLANT FROM TITANIUM AND ALLOYS THEREOF AND METHOD FOR PRODUCTION THEREOF (EMBODIMENTS) | 2023 |
|
RU2816023C1 |
METHOD FOR FABRICATION OF THREE-DIMENSIONAL BIOFILMS OF MICROORGANISMS | 2021 |
|
RU2769574C1 |
Authors
Dates
2013-06-10—Published
2011-12-28—Filed