FIELD: medicine.
SUBSTANCE: feces preparation for the DNA recovery is carried out by feces dissolution, homogenisation, filtration, fractionation, centrifugation and removal of a fecal tube. The feces are taken in an amount of 1000 mg, and a solvent is 0.1-1% acetic acid in an amount of 7 ml. The fractionation procedure is carried out using ethyl acetate in an amount of 2 ml, and the centrifuging is carried out at 1500 rpm for 1 minute.
EFFECT: invention improves the probability of PCT detection of opisthorchiasis even in the low content of opisthorchid fluke eggs in a clinical sample of faeces.
1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR BILE PREPARATION FOR DIAGNOSIS OF OPISTHORCHIASIS INVASION | 2013 |
|
RU2540477C2 |
| METHOD FOR PREDICTION OF INDIVIDUAL RISK OF BRONCHIAL ASTHMA IN REGIONS WITH HIGH AND LOW INCIDENCE OF HELMINTH INFECTIONS | 2012 |
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| METHOD OF DIAGNOSING LOW-INVASIVE TREMATODOSES OF HEPATOBILIARY SYSTEM | 2015 |
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RU2582946C1 |
| METHOD OF DIAGNOSIS OF ASSOCIATED OPISTHORCHIASIC INVASION IN PATIENTS WITH LIVER CIRRHOSIS | 2010 |
|
RU2442168C1 |
| METHOD FOR DIAGNOSING CHRONIC VIRAL B+C HEPATITIS ON THE OPISTORCHIASIS BACKGROUND | 2002 |
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| METHOD OF PREDICTING RISK OF BRONCHIAL ASTHMA DEVELOPMENT | 2008 |
|
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| METHOD FOR ISOLATION OF INTESTINAL VIRUS RNA FOR ANALYSIS BY RT-PCR METHOD | 2006 |
|
RU2313792C1 |
| METHOD FOR MYCOPLASMA HOMINIS DNA DETECTION FROM BLOOD SAMPLES | 2013 |
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RU2533238C1 |
| KIT FOR COMPLEX DIAGNOSTICS OF ENDOPARAZITES OF ANIMALS BY COPROSCOPY | 2016 |
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RU2683324C2 |
| METHOD FOR DETECTING LOW-COPY-NUMBER DNA OF NEISSERIA GONORRHOEAE IN LOW- AND ASYMPTOMATIC GONOCOCCAL INFECTIONS | 2019 |
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RU2716705C1 |
