METHOD FOR MYCOPLASMA HOMINIS DNA DETECTION FROM BLOOD SAMPLES Russian patent published in 2014 - IPC C07H21/04 G01N33/53 

FIELD: medicine.

SUBSTANCE: invention represents a method for the Mycoplasma hominis DNA detection from a blood sample by dividing it into a supernatant and precipitate by centrifugation followed by enriching the analysed material, recovering DNA by affine sorption with a sorbent and a lysis buffer added, incubating, washing the sorbent precipitate three times by centrifugation and removing the supernatant, drying the sorbent precipitate, adding a DNA elution solution and detecting by the PCR method. The method is characterised by the fact that the material to be analysed is a whole blood precipitate and to be enriched while recovering DNA by doubling up a number of test tubes per one sample with the whole blood precipitate 100 mcl and the sorbent 20 mcl in each; before the washing procedure, each test tube is added with the washing solution 375 mcl, while the content of the test tubes is combined in one test tube with adding the DNA elution solution 40 mcl to the sorbent precipitate.

EFFECT: higher diagnostic accuracy.

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