METHOD OF DNA SEPARATION Russian patent published in 2013 - IPC C12N15/10 B82B1/00 

FIELD: biotechnology.

SUBSTANCE: deoxyribonucleic acid (DNA) is separated from biological objects on carrier objects - gauze, paper, synthetic fabrics. The biological object - bone, horny tissue - is crushed. It is placed in a test tube containing a lytic buffer solution of the composition: 0.1 M Tris-HCl, 0.1 M EDTA, 0.1 M NaCl, 0.5% N-laurylsarcosil Na and proteinase K, pH 6-7. Cell lysate is obtained and is added with sorbent of magnetic nanoparticles of iron oxide Fe₃O₄, modified with chitosan. The mixture is stirred and incubated for 25-35 min. The test tube is placed on a magnetic rack and the mixture is divided into fractions - sorbent associated with DNA and the supernatant. The supernatant is removed. The residue is added with poured eluting buffer solution of the composition: 10 mM Tris-HCl, pH 7.4, 100 mM NaCl; 1 mM EDTA, and incubated. The test tubes are placed on a magnetic rack. The mixture is divided into fractions - sorbent - residue and supernatant - DNA, dissolved in the eluting buffer solution. The residue is removed. The final product of DNA is left in the supernatant.

EFFECT: invention enables to obtain DNA from different biological objects and increase the yield of the separated DNA not less than 1,5 times.