SET OF REACTIVES FOR DNA PURIFICATION Russian patent published in 2018 - IPC C12N15/10 

Abstract RU 2650865 C1

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry. Set of reagents for the purification of DNA from biological objects is proposed. Set includes lysis buffer solution, wash buffer solutions Nos. 1 and 2, eluting buffer solution. Lysis buffer solution contains guanidine thiocyanate, tris hydrochloride, tritone and sorbent, as a suspension from magnetic microspheres in saline solution. Wash buffer No. 1 contains guanidine thiocyanate, tris hydrochloride and ethyl alcohol. Wash buffer No. 2 contains tris hydrochloride, sodium chloride and ethyl alcohol. Eluting solution is deionized water.

EFFECT: invention provides an increase in DNA purification.

1 cl, 1 ex

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RU 2 650 865 C1

Authors

Viktorov Denis Aleksandrovich

Toropovskij Andrej Nikolaevich

Pimenov Sergej Vladimirovich

Nikitin Aleksej Georgievich

Dates

2018-04-17Published

2016-11-29Filed