FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry. Set of reagents for the purification of DNA from biological objects is proposed. Set includes lysis buffer solution, wash buffer solutions Nos. 1 and 2, eluting buffer solution. Lysis buffer solution contains guanidine thiocyanate, tris hydrochloride, tritone and sorbent, as a suspension from magnetic microspheres in saline solution. Wash buffer No. 1 contains guanidine thiocyanate, tris hydrochloride and ethyl alcohol. Wash buffer No. 2 contains tris hydrochloride, sodium chloride and ethyl alcohol. Eluting solution is deionized water.
EFFECT: invention provides an increase in DNA purification.
1 cl, 1 ex
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Authors
Dates
2018-04-17—Published
2016-11-29—Filed