METHOD FOR ASSESSING MORPHOFUNCTIONAL STATUS OF HUMAN THROMBOCYTES Russian patent published in 2013 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: sampled blood, or plasma, or thrombocyte concentrate is stained in a micro-tube with tripaflavine and acridine orange, and a fluorescence microscope is used to count thrombocyte granules, to measure a light intensity of 100-200 vitally stained cells; the light intensities are summed and divided on the thrombocyte count examined to calculate an average thrombocyte light intensity to be expressed in foot-candles per 1 cell relevant to one point characterising morphofunctional thrombocyte activity (MFAT). Further, the preparation with vitally stained thrombocytes is placed in a thermostat; a fluorescence microscope is used to count by spread-eagle thrombocytes with projecting granules, divided by total cell count analysed and expressed in %, wherein one percent is relevant to one point characterising thrombocyte adhesive activity (TAA). Then total MFAT and TAAT show the morphofunctional status of thrombocytes.

EFFECT: method provides an objective assessment of the morphofunctional status of human thrombocytes.

6 dwg, 6 tbl, 3 ex