EXPRESS METHOD FOR MORPHOFUNCTIONAL ANALYSIS OF PLATELETS, APPLICABLE FOR CLINICAL USE Russian patent published in 2017 - IPC G01N33/49 G01N21/91 

FIELD: medicine.

SUBSTANCE: invention relates to a method for morphofunctional analysis of platelets contained in platelet-rich plasma (PRP) or platelet concentrate (PC). The essence of the method is determination of platelets concentration (C_TP, ths/μl) in PRP or PC. The intravital staining of platelets is carried out by a dye prepared by dilution of 5-15 mg of trypaflavin and 15-25 mg of acridine orange at a room temperature in 100 ml of phosphate buffer at pH of 7.2-7.4 by introducing a dye into PRP or PC at a rate of 200 μl of dye per 1 ml of platelet concentrate or 100 μl of dye per 1 ml of platelet rich plasma. After that, a study of the preparation with stained platelets is performed using a fluorescence microscope followed by determination of the average luminescence intensity (LI_experiment) of the first field of view of the microscope, in addition, the theoretical luminous intensity (LI_theory) is determined according to the calibration curve or by the formula, reflecting the average luminescence intensity of the first field of view of the microscope with vitally stained cells _of the sample with a given platelet concentration (CTP), provided that all cells of this sample (100%) contain granules. Further, the relative content of platelets with granules (Dp.g.) is determined by the formula Dp.g.(%)=LI_experiment/LI_theory×100%, while the platelets suitable for clinical use are those having the value of Dp.g. of 35 to 75%.

EFFECT: application of the method allows to analyze the population of vitally stained cells with high accuracy.

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