METHOD FOR MAKING BRUCELLOSIS DIAGNOSTIC SERUM Russian patent published in 2015 - IPC A61K39/10 A61K39/40 A61B10/00 

FIELD: medicine.

SUBSTANCE: invention refers to veterinary science and diagnosis of infectious diseases, namely brucellosis. A method for making brucellosis diagnostic serum involves the single administration of a mixed antigen (100 billion cell of killed culture of the strain B.abortus 19) with the MONTANIDE™ ISA 61 VG adjuvant. _-On the 18-20^th day, the blood sample is taken, whereas the blood is taken twice from each producer animal at 16-20 ml of the blood per 1 kg of body weight, or the process bloodletting is performed on the 24-30^th post-hyperimmunisation day. The serum taken from each producer animal is poured separately into sterile bottles and preserved by adding 4% dry boric acid, heated on a water bath at 54-56°C for 40 minutes while stirring continuously, then placed for 10 days into a fridge at a temperature of 2-8°C. The blood sample is then checked for sterility, activity and specificity. The serum is expected to be sterile and have an agglutination titre not less than 1,000 International Units, and a complement binding titre of not less than 1:20.

EFFECT: technical effect is reducing the labour intensity of the process and the epidemic safety.

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