IMPROVED METHOD FOR PRODUCING DEAD CELL PREPARATIONS FOR FLUORESCENT IN SITU HYBRIDISATION Russian patent published in 2013 - IPC G01N33/48 

Abstract RU 2490635 C1

FIELD: medicine.

SUBSTANCE: invention may be used to produce dead cell preparations for fluorescent in situ hybridisation (FISH) of nucleic acids. That is ensured by applying 5 mcl of the suspension on a well bottom formed by a slide surface and 8 round holes of the diameter of 9 mm in a strip of parafilm 26×56 mm2 hermetically attached to the slide. It is spread along the glass slide for 3-5 sec with a roller on a heating table at temperature 60°C. The above strip along with the slide is heated at 95°C for 2 min and then torn off instantly from the slide.

EFFECT: higher quality of the slide washing, causes no increase of non-specific probe binding, provides uniform distribution of a contrast stain on the slide surface in FISH-examinations.

1 ex

Similar patents RU2490635C1

Title Year Author Number
METHOD OF PREPARING CELL PREPARATIONS FOR FLUORESCENT IN SITU HYBRIDISATION 2009
  • Ovsepjan Vanik Abramovich
RU2410663C1
METHOD OF PRODUCING CYTOGENETIC EPITHELIAL CELL PREPARATIONS FOR CARRYING OUT A FLUORESCENT IN SITU HYBRIDISATION REACTION 2019
  • Shkavrova Tatyana Gennadevna
  • Golub Elena Viktorovna
  • Mikhajlova Galina Fedorovna
  • Tsepenko Viktoriya Viktorovna
  • Ivanov Sergej Anatolevich
  • Kaprin Andrej Dmitrievich
RU2706220C1
METHOD FOR FLUORESCENT HYBRIDIZATION IN SITU USING THE FGFR1 DNA PROBE IN DIFFERENT MAMMALIAN SPECIES ON CYTOLOGICAL PREPARATIONS 2021
  • Dilekova Olga Vladimirovna
  • Mitenko Vasilisa Vasilevna
RU2755392C1
METHOD OF MARKING HUMAN 7TH CHROMOSOME IN METAPHASIC AND INTERPHASIC CELLS 2009
  • Jurov Jurij Borisovich
  • Aleksandrov Ivan Aleksandrovich
  • Solov'Ev Il'Ja Vladimirovich
  • Jurov Ivan Jur'Evich
  • Vorsanova Svetlana Grigor'Evna
RU2425890C2
RECOMBINANT PLASMID DNA pYAII-39, USED FOR 4TH AND 9TH HUMAN CHROMOSOME MARKING IN METAPHASIC AND INTERPHASIC CELLS 2006
  • Jurov Jurij Borisovich
  • Aleksandrov Ivan Aleksandrovich
  • Vorsanova Svetlana Grigor'Evna
  • Solov'Ev Il'Ja Vladimirovich
RU2325441C1
RECOMBINANT PLASMID DNA ALPHA-R-12 DESIGNATED FOR MARKING 6-TH HUMAN CHROMOSOME IN METAPHASE IN INTERPHASE CELLS 2004
  • Jurov Ju.B.
  • Jakovlev A.G.
  • Vorsanova S.G.
  • Solov'Ev I.V.
RU2265060C1
DIAGNOSTIC TECHNIQUE FOR UROTHELIAL BLADDER CANCER 2011
  • Vorobtsova Irina Evgen'Evna
  • Vasil'Eva Zaure Zhenisovna
RU2456607C1
METHOD FOR IDENTIFYING CHROMOSOMAL ABNORMALITIES BY THE FISH METHOD IN MULTIPLE MYELOMA 2021
  • Lapteva Yuliya Sergeevna
  • Ovsepyan Vanik Abramovich
RU2771933C1
METHOD FOR DETERMINING HYPERDIPLOIDY USING MORPHOMETRIC ANALYSIS IN MULTIPLE MYELOMA 2022
  • Ovsepyan Vanik Abramovich
  • Lapteva Yuliya Sergeevna
  • Dyakonov Dmitrij Andreevich
RU2789782C1
METHOD AND DEVICE FOR DETERMINING CHROMOSOME STRUCTURE 2005
  • Vallabkhaneni Ramesh
RU2471871C2

RU 2 490 635 C1

Authors

Ovsepjan Vanik Abramovich

Dates

2013-08-20Published

2012-04-12Filed