FIELD: medicine.
SUBSTANCE: invention may be used to produce dead cell preparations for fluorescent in situ hybridisation (FISH) of nucleic acids. That is ensured by applying 5 mcl of the suspension on a well bottom formed by a slide surface and 8 round holes of the diameter of 9 mm in a strip of parafilm 26×56 mm2 hermetically attached to the slide. It is spread along the glass slide for 3-5 sec with a roller on a heating table at temperature 60°C. The above strip along with the slide is heated at 95°C for 2 min and then torn off instantly from the slide.
EFFECT: higher quality of the slide washing, causes no increase of non-specific probe binding, provides uniform distribution of a contrast stain on the slide surface in FISH-examinations.
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Authors
Dates
2013-08-20—Published
2012-04-12—Filed