FIELD: medicine.
SUBSTANCE: method is implemented by detecting a marker in herparinised venous blood by incubating the blood with a mycobacterial antigen material (a test sample) and with normal saline (a reference); thereafter the blood is examined to measure CD45+ lymphocytes of the fluoroisothiocyanate-labelled markers; the fluorescence of the labelled CD45+ lymphocytes in the test sample in relation to the reference enables to consider that the individual has been infected with the very species of mycobacteria with the antigen material has been used for the blood incubation in a test tube.
EFFECT: method enables supporting for the fact of the mycobacterial infection, identifying the infection agent species, assessing the vaccination effectiveness; the method can be implemented for a relatively short time.
1 ex
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Authors
Dates
2013-08-27—Published
2011-12-26—Filed