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2 492 458

(13)

(51)

IPC

G01N27/26(2006-01-01)

G01N33/02(2006-01-01)

(21) (22)

Application

2012112496/28, 2012-03-30

(24)

Start date

2012-03-30

(22)

Actual filing date

2012-03-30

(45)

Published

2013-09-10

(72)

Inventor

Jakuba Jurij FedorovichNen'Ko Natalija IvanovnaFilimonov Mikhail Vasil'EvichShestakova Vera VladimirovnaZakharova Marina Vital'Evna

(73)

Holder

Gosudarstvennoe Nauchnoe Uchrezhdenie Severo-Kavkazskij Zonal'Nyj Nauchno-Issledovatel'Skij Institut Sadovodstva I Vinogradarstva Rossel'Khozakademii

METHOD OF DETERMINING GLUCOSE, SACCHAROSE AND FRUCTOSE Russian patent published in 2013 - IPC G01N27/26 G01N33/02

Abstract RU 2492458 C1

FIELD: chemistry.

SUBSTANCE: method involves multiple dilution of a sample to total sugar content of more than 10 g/dm³, centrifuging and conducting analysis on a capillary electrophoresis system in a quartz capillary with effective length of 0.5 m, inner diameter of 75 mcm, wherein according to the invention, analysis is conducted using a main electrolyte containing 4 g/dm³ potassium sorbate, 8 g/dm³ 10% aqueous solution of a cetyl-trimethyl-ammonium base (CTA-OH), 36 g/dm³ glycerine, 0.16 g/dm³ potassium hydroxide, with negative polarity of voltage and detection wavelength of 254 nm.

EFFECT: faster, improved and high objectivity of quantitative analysis of sugars.

1 tbl, 6 ex

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RU 2 492 458 C1

Authors

Jakuba Jurij Fedorovich

Nen'Ko Natalija Ivanovna

Filimonov Mikhail Vasil'Evich

Shestakova Vera Vladimirovna

Zakharova Marina Vital'Evna

Dates

2013-09-10—Published

2012-03-30—Filed