METHOD FOR MOLECULAR-GENETIC GROUPING OF STRAINS Bordetella pertussis Russian patent published in 2013 - IPC C12Q1/68 C12P19/30 C12N1/20 C12R1/01 

FIELD: biotechnologies.

SUBSTANCE: method includes extraction of DNA from strains B.pertussis, performance of a polymerase chain reaction with simultaneous introduction of specific primers PF-PR for production of amplicons of a fragment of a promotor of pertussis toxin ptxP, restriction of produced amplicons with endonucleases Kpnl and Mlul and electrophoresis of restriction products in agarose gel. Differentiation of strains B.pertussis is carried out by comparison of size of amplicons of DNA fragments of promotor ptxP of investigated strains B.pertussis with reference samples of DNA strains B.pertussis.

EFFECT: invention may be used for accelerated detection of epidemic strains with modified genetic structure during microbiological and molecular-genetic monitoring of Bordetella pertussis strains and for further development of promising diagnostic and prevention preparations on their basis.

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