FIELD: medicine.
SUBSTANCE: invention relates to field of medicine, namely to method of obtaining mineralised bone matrix. Method of obtaining mineralised bone matrix includes mechanical cleaning spongy bone from cartilage tissue and perforation of bone with pin. After that, bone is successively processed with solution of sodium chloride and twin-100 after mixing, with hydrogen peroxide solution, chloroform-ethanol mixture, with volume of all used solutions constituting not less than 5 volumes of spongy bone; after that, it is processed with alcohol-ether mixture, and mixed, all processes of mixing being carried out on magnetic mixer, then, centrifuged and aired, after which bone fragments are frozen, freeze-dried and sterilised under specified conditions.
EFFECT: claimed method makes it possible to reduce time of claimed method realisation, possible storage time of product obtained by claimed method is 3 years, with preservation by product of sterility and biological properties.
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Authors
Dates
2013-10-20—Published
2012-03-29—Filed